Fluorescently labeled targets were hybridized to agilent rice oligo microarrays for 17 h at 65c. Agilent technologies microarray scanner approved for in vitro diagnostics in china. Used agilent surescan g2600d microarray scanner g4900da. Agilent g2565ba microarray scanner iet refurbished. Agilent s surescan microarray scanner enables automated highspeed scanning and is able to produce highquality data from both legacy and upcoming arrays. Agilent surescan g2600d microarray scanner g4900da g2600d is part number of scanner itself, and g4900da is a system of surescan g2600d scanner and computer with software. Refurbished agilent g2565ca dna microarray scanner pn g2505c with surescan highresolution technology. This section describes the features that make the microarray scanner a powerful tool for agilents microarray analysis solution. Features special to this model are highlighted yellow. Notice that the feature is clearly distinguished on the agilent microarray scanner, but is barely distinguishable on the brand y scan, and is not detectable at all on the brand z scan. Contains three subtables, storing the feature extraction parameters, microarray level statistics, and featurelevel data.
Quantile normalization and differential analysis were performed with the agilent genespring software v. G2600d is part number of scanner itself, and g4900da is a system of surescan g2600d scanner and computer with software. Designed for research using microarrays, the scanner system provides a laserinduced fluorescence scanning solution designed to read microarrays printed on standard 1 inch by 3 inch glass slides. Agilent s reagent rental program offers a flexible and affordable solution for microarray analysis using the complete agilent platform. Agilent s widely used cgh array, followed by data analysis using a novel algorithm implemented within agilent s genomic workbench software. The surescan microarray scanner is a key component of agilents complete microarray solution.
There is a builtin ozone free environment to prevent the slides from photo bleaching. After hybridization, following wash processes were performed according to the manufacturers instructions, and hybridized microarrays were scanned using an agilent microarray scanner g2565ca. Hybridized microarray slides were scanned with an agilent dna microarray scanner g2505c and data was analyzed with the agilent scancontrol version 8. Refurbished agilent g2565ba microarray scanner pn g2505b complete with 48 position carousel, feature extraction and analysis software, and pc data system. The image on a chip was acquired with a g4900da surescan microarray scanner agilent technologies, ca, usa and analyzed with agilent cytogenomics software agilent technologies for chromosome gain or loss. Apr 12, 2018 after scanning the hybridized slides using the agilent g2565ca microarray scanner and scan control software 8. It measures the fluorescence intensity of two dyes simultaneously from labeled sample nucleic acid bound to microarrays. A crossplatform comparison of affymetrix and agilent. Agilent surescan microarray scanner the new agilent surescan microarray scanner is the foundation of our complete microarray solution and represents the latest innovation in. Evaluation of highresolution scanners for analyzing. Jan 10, 20 fluorescently labeled targets were hybridized to agilent rice oligo microarrays for 17 h at 65c. Both the limma and the agilp packages use the output for the red and green channels from the agilent feature exrtaction software as inputs, as far as i can tell, and i dont have them. I have been planing to use the bioconductor package to perform the analysis, but i am at a loss as to how to go about doing this.
Current listings 4396 labx member since 6th october, 2004 15 years ago seller location markham, ontario, canada shipping policy. Agilent s feature extraction software reads and processes up to 100 raw microarray image files in an automated, walkaway mode. Agilent s dna microarray scanner with surescan highresolution technology is the key component of microarray based applications in which increased coverage of the genome is a necessity. The probe intensities were extracted using the agilent feature extraction software, and. Tecans powerscanner is the microarray scanner of choice for highdensity dna microarrays and protein microarrays. Agilent surescan g2600d microarray fluorescence scanner. Data analysis software 2 perpetual licenses of agilent feature extraction included approximate scanner. Pdf preprocessing and differential expression analysis of. Agilent dna microarray scanner g2565ca table contains all key specifications for the dna microarray scanner, model ba.
The file lists the location of each probe in the design from back, left orientation along with its accession number, gene name, performance score, and other information. Data analysis cytogenomics software g1662aag1667aa agilent microarrays, reagents and scanner. Identification of novel differentially expressed lncrna and. Genomewide profiling to analyze the effects of high fat. Functional role of a novel long noncoding rna in esophageal. The surescan dx microarray scanner system is indicated for. Agilent 2100 bioanalyzer g2938c with control system and software condition. The probe intensities were extracted using the agilent feature extraction software, and probe intensities were background corrected and normalized quantile normalization using the limma package in r.
Microarray scanners are devices used to measure the fluorescent areas of a dna microarray to reveal information about the simultaneous activity of thousands of genes. The signal level of the feature shown is 8 counts and 58 counts over background in the red and green channels, respectively, on the agilent microarray scanner. Aberrations were detected by using default setting. Find great deals on a wide selection of microwave digesters, reagents, synthesis, and microarray scanners in auctions, and classified for sale ads on labx. The agilent surescan scanner provides a precise and efficient microarray scanner system for many research applications. The agilent dna microarray scanner is a 48slide scanning system enabled by surescan highresolution technology capable of scanning with 2, 3, 5 or 10 micron resolution that can read 1 x 3 glass slide microarrays and seamlessly feature extract them all using agilents feature extraction software with robust statistical analyses. The scanned tiff images were analyzed numerically and background corrected using the agilent feature extraction software version 10. System description in this section learn about the hardware and software features of the microarray scanner, the parts and system requirements. When buying a microarray scanner, important features to consider are sensitivity, resolution, scan area, rapidity of scan. Comparative analysis of naive, primed and ground state. Surescan microarray scanner for flexible analysis for genomics and cytogenetics microarrays.
The surescan dx microarray scanner is class ii, 510k exempt in the us. Identification of novel genetic markers for mouse yolk sac. Raw intensity data were obtained using agilent s feature extraction software version 10. Labeled crna was hybridized to a sureprint g3 mouse ge 8. Agilent technologies agilent g4900da surescan microarray scanner system microarray scan control software 9. Product dna microarray scanner with sure scan highresolution technology, model g2565ca. Agilent 2100 bioanalyzer g2939a with control system and software.
Agilent g2565ca microarray scanner system for sale. Its ability to correctly identify and focus on the arrayed surface of glass slides, including nitrocellulosecoated and reflective surfaces, makes it a uniquely versatile microarray scanner. The array slide was scanned using an agilent g2565ca microarray scanner system, and probe signals were quantified using agilent s feature extraction software version 9. Vector network analyzer vna software for the control of hp, agilent and keysight vector network analyzer, such as the 8753, 8720. If you will be using a 3rd party microarray scanner other than the axon genepix, the scanner s software will need this file to process the images. Surescan microarray scanner high sensitivity and resolution. Agilent g2505a dna sequencer micro array scanner ebay. The highresolution scanner released by agilent is the model with id number g2565ca which can scan highdensity microarrays up to 2 um resolution figure 1. The surescan dx microarray scanner system, consisting of surescan dx microarray scanner with autoloader and agilent microarray scan control software, is intended to measure fluorescence signals of labeled dna and rna target hybridized to microarrays. We ship our instruments immediately, delivery can take up to 4.
The agilent g2565ca microarray scanner is part of the microarray analysis solution from agilent technologies. The microarray scanner is a sophisticated laser induced fluorescence scanner designed to read microarrays. G2565ca feature description dynamic range 104 16bit data format, 105 20bit data format, 106 with xdr scanning dynamic autofocus continually adjusts scanners focus, keeping features in focus at all times autoloa. Agilent dna microarray scanner agilents dna microarray scanner with surescan highresolution technology is the key component of microarraybased applications in which increased coverage of the genome is a necess. The microarray scanner measures the fluorescence intensity of.
The scan control program communicates with the scanner. Microarray scanners microarray scanner systems biocompare. The image on a chip was acquired with a g4900da surescan microarray scanner agilent technologies, ca, usa and analyzed with agilent cytogenomics software agilent technologies for. Preprocessing and differential expression analysis of agilent microrna arrays using the agimicrorna bioconductor library. The microarray scanner is a sophisticated laserinduced fluorescence scanner designed to read microarrays printed on standard 1 in.
In the subsequent data preprocessing, raw intensity data from the spotted arrays and agilent arrays were treated equally. Product dna microarray scanner with sure scan highresolution technology, model g2565ca special features. Data were extracted on a g2565ca dna microarray scanner using the agilent feature extraction software agilent. Gse75471 osmyc2responsive genes in osmyc2overexpressing. Dna microarray scanner agilent g25265ca with sure scan high resolution technology. An economical tool for genetic screening of large global populations. The array slide was scanned using an agilent g2565ca microarray scanner system, and probe signals were quantified using agilents feature extraction software version 9. Agilent g2565ca microarray scanner system spectralab. The agilent surescan dx microarray scanner is cemarked for in vitro diagnostic ivd use in many countries outside the us. After hybridization and washing, the slides were scanned using an agilent g2565ca microarray scanner. Find agilent, tecan, and invitrogen for sale on machinio. Microarray design files available for download agilent. Please contact us for complete warranty, service contract and usedrefurbished agilent g2565 ca. Agilent microarray scanner and feature extraction software.
Guida alluso dello scanner per microarray agilent g2565ca software scan control 8. Data were extracted on a g2565ca dna microarray scanner using the agilent feature extraction software agilent technologies with default settings. The software automatically finds and places microarray grids, rejects outlier pixels, accurately determines feature intensities and ratios, flags outlier pixels, and calculates statistical confidences. The agilent dna microarray scanner is a 48slide scanning system enabled by surescan highresolution technology capable of scanning with 2, 3, 5 or 10 micron resolution that can read 1 x 3 glass slide microarrays and seamlessly feature extract them all using agilent s feature extraction software. This software is usable only with the agilent highresolution scanner g2565ca or highresolution scanner upgrade. Rna was applied microarray agilent technologies, 8. Agilent g2565ca microarray scanner system scan control software 8.
Output format of agilent feature extraction and agilent genomic workbench agw software. Surescan microarray scannerg4900da from agilent technologies description agilents surescan microarray scanner enables automated highspeed scanning and is able to produce highquality data from both legacy and upcoming arrays. Using data from 243 breast cancer samples analyzed on two different microarray platforms, our inhouse 29k array platform and agilent sureprint g3 microarray platform, we. Jul 03, 2014 the scanned images of the spotted arrays were quantified by gene pix pro 6. File format used for the storage and exchange of microarray data and metainformation. The microarray scanner is a sophisticated laser induced fluorescence scanner designed to read microarrays printed on standard 1 in x 3 in slides. The surescan microarray scanner is a laser induced fluorescence scanner designed to read microarrays printed on a standard 1 inch x 3 inch glass slides. Microarray scanners highquality array scanning systems. The agilent dna microarray scanner is a 48slide scanning system enabled by surescan highresolution technology capable of scanning with 2, 3, 5 or 10 micron resolution that can read 1 x 3 glass slide microarrays and seamlessly feature extract them all using agilents feature extraction software. It includes the surescan microarray scanner, software and accessories, the g2565 series microarray scanner software and accessories.
Arrays were scanned with an agilent g2565ca microarray scanner system agilent, santa clara, ca. Dna microarray scanner dna microarray scanner g2565ca. Whether performing gene expression studies, array cgh, mirna pro. Surescan microarray scannerg4900da from agilent technologies.
The microarrays were scanned using an agilent g2565ca microarray scanner with the scan control software v8. G2565 series microarray scanner software and accessories. Surescan dx microarray scanner by agilent technologies, inc. We carry used and preowned dna sequencing systems and agilent bioanalyzers. Agilent microarray scan control icon when the program starts, the agilent microarray scan control program main window opens and the scanner performs its initialization sequence. The scanned tiff images were analyzed numerically and background corrected using the agilent feature extraction software. Please contact us for complete warranty, service contract and usedrefurbished agilent g2565 ca system details. By agreeing to purchase a predefined volume of microarrays and reagents over the course of a specified agreement period, agilent will loan your lab a complete system including the scanner, hybridization oven, hybridization chambers, and software. The scanner is equipped with new optics, electronics, and software. Agilent g2565ca dna microarray scanner iet refurbished. Here is an agilent surescan g2600d microarray scanner g4900da. Scan control software is the user interface software that controls the agilent scanner. Agilent technologies 11 surescan microarray scanner system microarray scan control software 9. Data analysis cytogenomics software g1662aag1667aa agilent microarrays, reagents and scanner are.
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